The Trail de l'Aqueduc begins in Cours, a village in France's Lot department, crossing the Causses du Quercy Regional Natural Park. This park, a UNESCO Global Geopark spanning over 185,500 hectares, offers a vast limestone landscape for the event, rather than a small woodland setting. The race typically takes place in late January, a time when icy winds are common. Participants can select between a 13 km trail featuring 580 m of elevation gain and a more demanding 25 km route with 1,250 m of climbing.
The race's name originates from a segment passing through a section of an aqueduct beneath the rock, necessitating a headlamp. The course also involves crossing the Nouaillac stream and navigating rough paths with mud, stones, cliffside sections, numerous ascents and descents, and some rope-assisted passages. The shorter race attracts a larger number of participants, with over 200 runners in a past edition, while the 25 km has seen more than 100. Nearby Cahors offers post-race dining options, featuring regional specialties such as black truffles, Rocamadour cheese, and Cahors wine.