The TT City Run begins on the TT Circuit and concludes in Assen's center, connecting the city’s motor-racing venue with its festival streets. Participants can select from 5.4 km, 10 km, or 14.6 km distances. The event inaugurates the TT Festival, integrating it with Assen’s broader TT week instead of functioning as an independent road race.
The primary courses all lead from the circuit towards the city center. Meanwhile, children compete over shorter distances within Assen: 500 meters for the youngest participants and 1.2 km for older children. The schedule also features TT Circuit Laps, allowing individuals to traverse the circuit at their own speed, accommodating those using mobility aids. Additionally, there is a Business Run for teams and a College Run for local students. All finishers are awarded a TT City Run medal.